Improvement of chilled seabass sperm conservation using a cell culture medium
Identifieur interne : 000024 ( France/Analysis ); précédent : 000023; suivant : 000025Improvement of chilled seabass sperm conservation using a cell culture medium
Auteurs : C. Fauvel [France] ; S. Boryshpolets [République tchèque] ; J. Cosson [République tchèque] ; J. G. Wilson Leedy [États-Unis] ; C. Labbé [France] ; P. Haffray [France] ; M. Suquet [France]Source :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2012-12.
Abstract
The sperm of seabass is very fragile and it quickly loses its ability to fertilize after collection either if kept undiluted or in classic saline media. In order to avoid cryopreservation when only short conservation is required, the process of sperm management including sperm collection, sperm dilution rate in storage medium and storage medium composition, was subject to experimental trials. A concentration of 20% urine generated a low pH of seminal fluid, and it immediately altered the motility ability. However, pH did not seem to be the key agent of motility prevention since sperm dilution in Leibovitz culture medium (L15) or in classic saline medium both presenting a similar low pH (7.3) did not affect motility. L15 increased the duration of sperm survival by 2 days at 4°C after collection. Moreover, dilution could be restricted to 1 : 3 (v : v) for conservation of chilled sperm. Chilled sperm could be cryopreserved with no more damages than those observed after freezing of fresh sperm.
Url:
DOI: 10.1111/jai.12071
Affiliations:
- France, République tchèque, États-Unis
- District fédéral central, Languedoc-Roussillon, Occitanie (région administrative), Région Bretagne
- Moscou, Palavas, Rennes
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ISTEX:5413E02DE5C303416ED8330BBDA977E4B53E2BEALe document en format XML
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Wilson Leedy</name><affiliation wicri:level="3"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biological Sciences, University of Idaho, Center for reproductive biology, ID, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Labbe, C" sort="Labbe, C" uniqKey="Labbe C" first="C." last="Labbé">C. Labbé</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INRA, UR 1037 Scribe, Rennes</wicri:regionArea><placeName><region type="region">Région Bretagne</region><region type="old region">Région Bretagne</region><settlement type="city">Rennes</settlement></placeName></affiliation></author><author><name sortKey="Haffray, P" sort="Haffray, P" uniqKey="Haffray P" first="P." last="Haffray">P. 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In order to avoid cryopreservation when only short conservation is required, the process of sperm management including sperm collection, sperm dilution rate in storage medium and storage medium composition, was subject to experimental trials. A concentration of 20% urine generated a low pH of seminal fluid, and it immediately altered the motility ability. However, pH did not seem to be the key agent of motility prevention since sperm dilution in Leibovitz culture medium (L15) or in classic saline medium both presenting a similar low pH (7.3) did not affect motility. L15 increased the duration of sperm survival by 2 days at 4°C after collection. Moreover, dilution could be restricted to 1 : 3 (v : v) for conservation of chilled sperm. Chilled sperm could be cryopreserved with no more damages than those observed after freezing of fresh sperm.</div></front></TEI><affiliations><list><country><li>France</li><li>République tchèque</li><li>États-Unis</li></country><region><li>District fédéral central</li><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li><li>Région Bretagne</li></region><settlement><li>Moscou</li><li>Palavas</li><li>Rennes</li></settlement></list><tree><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Fauvel, C" sort="Fauvel, C" uniqKey="Fauvel C" first="C." last="Fauvel">C. Fauvel</name></region><name sortKey="Fauvel, C" sort="Fauvel, C" uniqKey="Fauvel C" first="C." last="Fauvel">C. Fauvel</name><name sortKey="Haffray, P" sort="Haffray, P" uniqKey="Haffray P" first="P." last="Haffray">P. Haffray</name><name sortKey="Labbe, C" sort="Labbe, C" uniqKey="Labbe C" first="C." last="Labbé">C. Labbé</name><name sortKey="Suquet, M" sort="Suquet, M" uniqKey="Suquet M" first="M." last="Suquet">M. Suquet</name></country><country name="République tchèque"><noRegion><name sortKey="Boryshpolets, S" sort="Boryshpolets, S" uniqKey="Boryshpolets S" first="S." last="Boryshpolets">S. Boryshpolets</name></noRegion><name sortKey="Cosson, J" sort="Cosson, J" uniqKey="Cosson J" first="J." last="Cosson">J. Cosson</name></country><country name="États-Unis"><region name="District fédéral central"><name sortKey="Wilson Leedy, J G" sort="Wilson Leedy, J G" uniqKey="Wilson Leedy J" first="J. G." last="Wilson Leedy">J. G. Wilson Leedy</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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